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Polymerase chain reaction detection of Pasteurella multocida type B:2 in mice following oral inoculation

Abdullah, Faez Firdaus Jesse and Osman, Abdinasir Yusuf and Adamu, Lawan and Mohd Yusof, Mohd Syamil and Omar, Abdul Rahman and Saharee, Abdul Aziz and Haron, Abdul Wahid and Abdullah, Rasedee and Saad, Mohd Zamri (2013) Polymerase chain reaction detection of Pasteurella multocida type B:2 in mice following oral inoculation. Asian Journal of Animal and Veterinary Advances, 8 (3). pp. 493-501. ISSN 1683-9919; ESSN: 1996-3289

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Abstract

Haemorrhagic Septicaemia (HS) is an acute, fatal, septicaemic disease of cattle and water buffaloes caused by Pasteurella multocida, serotype B:2 in tropical countries. The limitations associated with accurate predictions of mortality, survival levels and the detection of the presence of the organism from various organs of infected animals. Hence, this study used mouse model to evaluate the pattern of mortality and bacterial recovery from organs. Twenty-four mice were randomly divided into two groups. Infected group were inoculated orally with 109 colony forming unit of P. multocida type B, the group 2 were negative controls. The mice were observed for 5 days post-inoculation. At necropsy, visceral organs of dead animals were subjected for the confirmation using Polymerase Chain Reaction (PCR). The results showed that mortality rate was significantly different (p<0.05) between the infected and control groups. Within infected group, highly significant difference (p<0.05) was observed where 12.5% of the mortality rate was recorded within 24 h and 62.5% within 48 h post-infection. The survival rate, in infected group, was found to be around 25%. In diagnosis, P. multocida type B was detected from all organs of animals that did not survive. In contrast, P. multocida type B was neither recovered nor detected from the organs of mice which survived until the end of the experimental period (120 h). The results of this study indicated that manipulation of the organism in experimental animals provided clear information of the incidence of the disease in the field.

Item Type:Article
Keyword:Pasteurella multocida type B; Oral inoculation; Polymerase chain reaction; Mice; Organs
Faculty or Institute:Faculty of Veterinary Medicine
Publisher:Academic Journals
DOI Number:10.3923/ajava.2013.493.501
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/ajava.2013.493.501
ID Code:29836
Deposited By: Nabilah Mustapa
Deposited On:04 Jul 2015 09:40
Last Modified:11 Feb 2016 14:13

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