Effects of Dehydration on Freezing Characteristics and Survival in Liquid Nitrogen of Three Recalcitrant Seeds

Hor, Yue Luan and Stanwood, P. C. and Chin, H. F. (1990) Effects of Dehydration on Freezing Characteristics and Survival in Liquid Nitrogen of Three Recalcitrant Seeds. Pertanika, 13 (2). pp. 309-314.

[img] PDF
1594Kb

Abstract

The recalcitrant seeds rambutan( Nephelium lappaceum). durian (Durio zibethinus) and cempedak (Artocarpus inleger) have a high critical moisture content (below which ·rapid loss of viability occurs of 27.0%, 26.0% and 37.9%,respectively. The critical moisture for embroys were higher at 39.0% for rambutan, 53.9% for durian and 43.2% for Cempedak. Differential Thermal analysis of the embroyos confirmed that their threshhold moistures (below which there is no freezable water) were lower than their critical moistureS. The Threshhold moistures for rambutan, durian and cempedak embryos were approximately 30%, 32% and 33% respectively. It is suggested that unsuccessful attempts at cryopreservation of embroyos of 'recalcitrant seeds in the past maybe due to the absence of safe window between the high critical moisture content and the threshhold moisture. This results in freezing injury at the higher moistures and dehydration injury' at the lower moistures. Potential techniques to overcome this and improve cryopreservation of recalcitrant seed embryos are discussed.

Item Type:Article
Keyword:recalcitrant seeds, cryopreservation
Faculty or Institute:Faculty of Agriculture
ID Code:2813
Deposited By: Nur Izyan Mohd Zaki
Deposited On:18 Nov 2009 02:16
Last Modified:27 May 2013 07:03

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 18 Nov 2009 02:16.

View statistics for "Effects of Dehydration on Freezing Characteristics and Survival in Liquid Nitrogen of Three Recalcitrant Seeds"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.