Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.
Ahmad Tajudin, Asilah and Petersson, K. and Lenshof, A. and Sward-Nilsson, A. M. and Aberg, L. and Marko-Varga, G. and Malm, J. and Lilja, H. and Laurell, T. (2013) Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples. Lab on a Chip, 13 (9). pp. 1790-1796. ISSN 1473-0197; ESSN: 1473-0189
On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%.
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