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Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.

Ahmad Tajudin, Asilah and Petersson, K. and Lenshof, A. and Sward-Nilsson, A. M. and Aberg, L. and Marko-Varga, G. and Malm, J. and Lilja, H. and Laurell, T. (2013) Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples. Lab on a Chip, 13 (9). pp. 1790-1796. ISSN 1473-0197; ESSN: 1473-0189

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Abstract

On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%.

Item Type:Article
Keyword:Point-of-care diagnostics; Immunoaffinity; PSA; Microfluidic; Acoustic.
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Royal Society of Chemistry
DOI Number:10.1039/c3lc41269e
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1039/c3lc41269e
ID Code:28121
Deposited By: Umikalthom Abdullah
Deposited On:28 May 2014 16:53
Last Modified:14 Sep 2015 15:55

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