Goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation.
Al-Qubaisi, Mothanna and Rosli, Rozita and Subramani, Tamilselvan and Omar, Abdul Rahman and Yeap, Swee Keong and Ali, Abdul Manaf and Mohamed Alitheen, Noorjahan Banu (2013) Goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation. Natural Product Research, 27 (23). pp. 2216-2218. ISSN 1478-6419
oniothalamin is a biologically active styrylpyrone derivative isolated from various Goniothalamus species. The ability of goniothalamin to induce apoptosis via caspase-3 activation against hepatoblastoma (HepG2) and normal liver cells (Chang cells) was studied using morphological and biochemical evaluations. HepG2 and Chang cells were treated with goniothalamin for 72 h and analysed by TUNEL and Annexin-V/PI staining. Furthermore, the post-mitochondrial caspase-3 was quantified using ELISA. In view of our results, goniothalamin induced apoptosis on treated cells via alteration of cellular membrane integrity and cleavage of DNA. On the other hand, post-mitochondrial caspase-3 activity was significantly elevated in HepG2 cells treated with goniothalamin after 72 h. These findings suggest that goniothalamin induced apoptosis on HepG2 liver cancer cells via induction of caspase-3 with less sensitivity on the cell line of Chang cells.
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