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A method for purification of intracellular protein.

Tey Beng Ti. (2009) A method for purification of intracellular protein.

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The present specification discloses an integrated purification technique for purifying recombinant intracellular protein (GFP) from intact E. coli cells using preparative PAGE column. Under the influence of electric fields, the negatively charged GFP was drifted out from cells without the need of cell disruption process. The electrophoresed cells were observed under SEM and some on the membrane of cells were observed. GFP was then separated from other host cells host protein along polyacrylamide gel and finally eluted electrophoretically from the gel. The invention has successfully recovered the GFP with purity and yeild 95% and 82%, respectively in 100 min operation.

Item Type:Patent
Keyword:Purifying recombinant intracellular protein; Purification technique; Cell.
Faculty or Institute:Faculty of Engineering
ID Code:27688
Deposited By: Nurul Ainie Mokhtar
Deposited On:02 Apr 2014 10:37
Last Modified:02 Apr 2014 10:37

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