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Method for isolating DNA

Mohd Ali Hassan. (2008) Method for isolating DNA.

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The present invention provides a method for the isolation of nucleic acid from microbial cells in an environmental sample. The method includes preparing a suspension of the environmental sample, lysing the suspended sample with a buffered solution, adding sodium dodecylsufate solution to the lysed suspended sample, carrying out solvent extraction and separation to obtain an aqueous phase, reacting the aqueous phase with solvents to generate an insoluble precipitate containing nucleic acid, and isolating the nucleic acid therefrom, thereby releasing high molecular weight nucleic acid pellets from the cells.

Item Type:Patent
Keyword:Isolating DNA; DNA; Nucleic acid
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
ID Code:26962
Deposited By: Nurul Ainie Mokhtar
Deposited On:04 Dec 2013 10:34
Last Modified:08 Sep 2016 10:37

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