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Isolation and identification of Brucella melitensis in goats.

Ahmed Al-Garadi, Maged and Bejo, Siti Khairani and Omar, Abdul Rahman and Zakaria , Zunita (2011) Isolation and identification of Brucella melitensis in goats. Journal of Animal and Veterinary Advances, 10 (8). pp. 972-979. ISSN 1680-5593

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Isolation of Brucella melitensis is the standard gold of identification and confirmation of animal brucellosis. However in Malaysia, Brucella sp., infection of goat was increasing recently and there is no evidence for diagnosis of the serover of Brucella sp., that cause the disease in goat population except the detection of serological methods. Isolation and identification of Brucella melitensis have been done by bacteriological methods in addition to conventional Polymerase Chain Reaction (PCR) and Real-time PCR for detection of Brucella melitensis from samples collected from vaginal swabs on suspected farm. In conclusion, four isolate have been got out of 300 vaginal samples and all isolate is belong to Brucella melitensis server 1. The Real-time PCR is the easy and save method for confirmation of brucellosis in goats population.

Item Type:Article
Keyword:Goats; Brucellosis; Isolation; Real-time PCR.
Faculty or Institute:Faculty of Veterinary Medicine
Publisher:Medwell Publishing
DOI Number:10.3923/javaa.2011.972.979
ID Code:25366
Deposited By: Nur Farahin Ramli
Deposited On:22 Oct 2013 08:27
Last Modified:09 Oct 2015 15:25

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