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Detection of brucella melitensis in blood samples collected from goats

Al-Garadia, Maged Ahmed and Bejo, Siti Khairani and Zakaria, Zunita and Omar, Abdul Rahman (2011) Detection of brucella melitensis in blood samples collected from goats. Journal of Animal and Veterinary Advances, 10 (11). pp. 1437-1444. ISSN 1680-5593

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Official URL: http://dx.doi.org/10.3923/javaa.2011.1437.1444

Abstract

PCR assays have been shown to be a promising option for the diagnosis of brucellosis. However, there is no study conducted in Malaysia to identify the brucellosis in goat’s population. In this study three whole blood samples and sera were collected from goat’s farm in Kedah state Malaysia which was suspected to have brucellosis. Serological and molecular detection of brucellosis have been done including RBPT, CFT, conventional PCR and Real time. The evaluation of each test have been discussed rather than the sensitivity and specificity of the each test which can be used in Malaysia national eradication programs. In conclusion, the combination between the serological test and molecular technique specially real time PCR depend on IS711 region in hypothetical protein is promising and can be reduced to false positive result which can cause heavy economical loss during controlling programs.

Item Type:Article
Keyword:Goats; Brucellosis; CFT; RBPT; PCR
Faculty or Institute:Faculty of Veterinary Medicine
DOI Number:10.3923/javaa.2011.1437.1444
ID Code:25365
Deposited By: Nur Farahin Ramli
Deposited On:12 Jul 2013 11:42
Last Modified:11 Feb 2016 10:32

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