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Prevalence of adhesion and regulation of biofilm-related genes in different clones of staphylococcus aureus.


Atshan, Salman Sahab and Nor Shamsudin, Mariana and Sekawi, Zamberi and Than, Leslie Thian Lung and Hamat, Rukman Awang and Karunanidhi, A. and Ali, AlreshidiMateg and Ghaznavi-Rad, Ehsanollah and Ghasemzadeh-Moghaddam, Hamed and Chong, Pei Pei and Nathan , J.J. and Chong , Seng J.S. (2012) Prevalence of adhesion and regulation of biofilm-related genes in different clones of staphylococcus aureus. Journal of Biomedicine and Biotechnology, 2012 (976972). pp. 1-10. ISSN 1110-7243; ESSN:1110-7251

Abstract / Synopsis

Clinical information about genotypically different clones of biofilm-producing Staphylococcus aureus is largely unknown. We examined whether different clones of methicillin-sensitive and methicillin-resistant S. aureus (MSSA and MRSA) differ with respect to staphylococcal microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) in biofilm formation. The study used 60 different types of spa and determined the phenotypes, the prevalence of the 13 MSCRAMM, and biofilm genes for each clone. The current investigation was carried out using a modified Congo red agar (MCRA), a microtiter plate assay (MPA), polymerase chain reaction (PCR), and reverse transcriptase polymerase chain reaction (RT-PCR). Clones belonging to the same spa type were found to have similar properties in adheringto thepolystyrene microtiter plate surface. However, their ability to produce slime on MCRA medium was different. PCR experiments showed that 60 clones of MSSA and MRSA were positive for 5 genes (out of 9 MSCRAMM genes). icaADBC genes were found to be present in all the 60 clones tested indicating a high prevalence, and these genes were equally distributed among the clones associated with MSSA and those with MRSA. The prevalence of other MSCRAMM genes among MSSA and MRSA clones was found to be variable. MRSA and MSSA gene expression (MSCRAMM and icaADBC) was confirmed by RT-PCR.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: 10.1155/2012/976972
Publisher: Hindawi Publishing Corporation
Keywords: MSSA and MRSA; Biofilm-related genes; Staphylococcus aureus.
Depositing User: Nur Farahin Ramli
Date Deposited: 13 Sep 2013 10:06
Last Modified: 29 Sep 2015 09:23
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