Keyword Search:

Bookmark and Share

Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli

Al-Hetar, Khadega Yahya Abdullah and Sekawi, Zamberi and Shamsudin, Mariana Nor and Neela, Vasantha Kumari (2011) Molecular characterization of extended-spectrum beta-lactimase (ESBL) producing extra-intestinal pathogenic Escherichia coli. African Journal of Microbiology Research, 5 (31). art. no. 5E0281D27330. pp. 5662-5668. ISSN 1996-0808

[img] PDF
Restricted to Repository staff only


Official URL:


Despite the frequent isolation of extended-spectrum beta-lactamase producing Escherichia coli from extra-intestinal infections (ESBL-ExPEC), the virulent properties or the antibiotic resistance mechanisms or its phylogenetics is rarely studied in Malaysia. In the present investigation, we characterized 95 clinical isolates of ESBL-ExPEC collected over a one year period time from a tertiary hospital through Clermont dichotomous phylogenetic grouping, virulent gene profiling and ESBL production mechanisms. The highest frequency of E. coli isolation was observed among urine (40% / n=38), while the lowest was among tissue and peritoneal dialyses fluid (1.05% / n= 1). Phylogenetic grouping revealed that 42 (44.21%) isolates belonged to group D, while 26 (27.36%), 14(14.73%) and 13 (13.68) belonged to A, B2 and B1. All isolates were multi drug resistant, whereby 58.9% harboured CTX-M-1 and 22.10% CTX-M-9. None of the isolates carried CTX-M-2 or CTX-M-8 clusters. Eight (7.6%) isolates were positive for blaTEM-1 and two (1.9%) produced SHV-12 beta-lactamase. The CTX-M-9 cluster was found to be significantly (P=0.005) associated with pathogenic group. Majority of the isolates (48.42%) carried class 1 integrons, while 3 (3.15%) isolates showed positive for class 2-encodedintI2 integrase and no signal for class 3 integron. Virulence factors profiling identified iutA56 (58.94%) and kpsMII33 (34.73%) as frequent pathogenic determinants. Virulence studies on Caenorhabditis elegans showed that antibiotic susceptible E. coli strains have short life span than pathogenic or commensal ones. In conclusion, it is found that commensals are becoming multiple drug resistant and carries several virulent genes which warn the spread of drug resistance or virulent factors to the normal flora.

Item Type:Article
Keyword:Escherichia coli; ESBL; ESBL-ExPEC; blaTEM; blaSHV; CTX-M; Aenorhabditis elegans; Malaysia
Faculty or Institute:Faculty of Medicine and Health Science
Institute of Bioscience
Publisher:Academic Journals
ID Code:24703
Deposited By: Nur Farahin Ramli
Deposited On:10 Sep 2013 12:07
Last Modified:17 Nov 2017 10:05

Repository Staff Only: Edit item detail