Effect of organic-phase solvents on physicochemical properties and cellular uptake of astaxanthin nanodispersions.
Anarjan, Navideh and Tan, Chin Ping and Tau, Chuan Ling and Kwan, Liang Lye and Malmiri, Hoda Jafarizadeh and Nehdi, Imededdine Arbi and Cheah, Yoke Kqueen and Mirhosseini, Hamed and Sham Baharin, Badlishah (2011) Effect of organic-phase solvents on physicochemical properties and cellular uptake of astaxanthin nanodispersions. Journal of Agricultural and Food Chemistry, 59 (16). pp. 8733-8741. ISSN 0021-8561
Official URL: http://dx.doi.org/10.1021/jf201314u
A simplex centroid mixture design was used to study the interactions between two chosen solvents, dichloromethane (DCM) and acetone (ACT), as organic-phase components in the formation and physicochemical characterization and cellular uptake of astaxanthin nanodispersions produced using precipitation and condensation processes. Full cubic or quadratic regression models with acceptable determination coefficients were obtained for all of the studied responses. Multiple-response optimization predicted that the organic phase with 38% (w/w) DCM and 62% (w/w) ACT yielded astaxanthin nanodispersions with the minimum particle size (106 nm), polydispersity index (0.191), and total astaxanthin loss (12.7%, w/w) and the maximum cellular uptake (2981 fmol/cell). Astaxanthin cellular uptake from the produced nanodispersions also showed a good correlation with their particle size distributions and astaxanthin trans/cis isomerization ratios. The absence of significant (p > 0.05) differences between the experimental and predicted values of the response variables confirmed the adequacy of the fitted models.
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