In Vitro Fermentation of Mixed Rumen Microorganisms on Molasses-based Substrates

Abdullah, NorHani (1986) In Vitro Fermentation of Mixed Rumen Microorganisms on Molasses-based Substrates. Pertanika, 9 (3). pp. 331-337.

[img] PDF
4Mb

Abstract

A fermentor consisting of two compartments (called inner and outer) separated by a dialysis membrane was developed for studying the digestion pattern in vitro of rumen microorganisms of sheep fed a molasses-urea based diet. A bout 35 ml of strained rumen liquor withdrawn from Merino weathers adapted to a molasses-urea diet (100 g oaten chaff and molasses containing 3% urea and 0.135% mineral mix fed ad libitum) was used as the initial fermenting inoculum in the inner compartment. Molasses-urea substrate (8.333% molasses and 0.277% urea, w/v) prepared in artificial saliva was infused at 3 ml h -I into the fermenting medium and artificial saliva at 30 ml h - I into the outer compartment. Incubation was carried out in a specially constructed water bath maintained at 39°C and filled with CO gas. With these flow rates, pH of fermenting fluids was maintained within 2 •physiological range with volatile fatty acids (VFA) production at 30 - 40 J.J.mole ml- 1 h -I. Using the fermentor, the effect of partial substitution of tryptone for urea as a nitrogen source on rumen microorganisms adapted to a molasses-urea based diet was studied with infusion solution consisting of 8.333% molasses, 0.208% urea and 0.202% tryptone (w/v) prepared in artificial saliva. Factors related to cell yield studied were, VFA and ammonia (NH) productions, dry matter (DM) and CP yields. However, no positive effects of protein supplementation (when compared to molasses-urea substrate) were observed in VFA productions, DM and CP yields. Only NH 3 levels of fermentors infused with molasses-urea substrate were significantly higher (P<0.05) than protein supplemented fermentors.

Item Type:Article
Keyword:In vitro fermentation; molasses-urea-protein substrates; rumen microorganisms.
ID Code:2408
Deposited By: Nur Izyan Mohd Zaki
Deposited On:13 Nov 2009 02:09
Last Modified:27 May 2013 07:00

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 13 Nov 2009 02:09.

View statistics for "In Vitro Fermentation of Mixed Rumen Microorganisms on Molasses-based Substrates"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.