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Extraction of microsomal fraction from midguts of western spruce budworm Choristoneura occidentalis F., and rat liver, and their ability to expoxidize aldrin


Citation

Mohammad, Rosli (1985) Extraction of microsomal fraction from midguts of western spruce budworm Choristoneura occidentalis F., and rat liver, and their ability to expoxidize aldrin. Pertanika, 8 (2). pp. 231-236. ISSN 0126-6128

Abstract / Synopsis

Microsomal fractions were isolated from midguts of western spruce budworm larvae and rat liver. The microsomal preparations were then assayed for cytochrome P-450 content and aldrin epoxidase activity. Both microsomal preparations showed distinct spectrum of cytochrome P-450. Relative to protein content, the cytochrome P-450 content of midguts of western spruce budworm was lower by 7 - 9 times compared to that of the rat liver. Epoxidase activity is present in the two microsomal preparations as indicated by their ability to convert aldrin to dieldrin. The activity of both microsomal preparations was inhibited by piperonyl butoxide.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Publisher: Universiti Pertanian Malaysia
Keywords: Microsomal fractions; Western spruce budworm; Choristoneura occidentalis F.; Rat liver; Epoxidation; Aldrin
Depositing User: Nur Izzati Mohd Zaki
Date Deposited: 13 Nov 2009 08:37
Last Modified: 09 Mar 2015 14:32
URI: http://psasir.upm.edu.my/id/eprint/2366
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