Extraction of Microsomal Fraction from Midguts of Western Spruce Budworm Choristoneura occidentalis F., and Rat Liver, and their Ability to Expoxidize Aldrin

Mohammad, Rosli (1985) Extraction of Microsomal Fraction from Midguts of Western Spruce Budworm Choristoneura occidentalis F., and Rat Liver, and their Ability to Expoxidize Aldrin. Pertanika, 8 (2). pp. 231-236.

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Abstract

Microsomal fractions were isolated from midguts of western spruce budworm larvae and rat liver. The microsomal preparations were then assayed for cytochrome P-450 content and aldrin epoxidase activity. Both microsomal preparations showed distinct spectrum of cytochrome P-450. Relative to protein content, the cytochrome P-450 content of midguts of western spruce budworm was lower by 7 - 9 times compared to that of the rat liver. Epoxidase activity is present in the two microsomal preparations as indicated by their ability to convert aldrin to dieldrin. The activity of both microsomal preparations was inhibited by piperonyl butoxide.

Item Type:Article
Keyword:Microsomal fractions, western spruce budworm, Choristoneura occidentalis F., rat liver, epoxidation, aldrin
Faculty or Institute:Faculty of Agriculture
ID Code:2366
Deposited By: Nur Izzati Mohd Zaki
Deposited On:13 Nov 2009 00:37
Last Modified:27 May 2013 07:00

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