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Utilization of oil palm decanter cake for cellulase and polyoses production

Abdul Razak, Mohamad Nafis and Ibrahim, Mohamad Faizal and Phang, Lai Yee and Hassan, Mohd Ali and Abd. Aziz, Suraini (2012) Utilization of oil palm decanter cake for cellulase and polyoses production. Biotechnology and Bioprocess Engineering, 17 (3). pp. 547-555. ISSN 1226-8372; ESSN: 1976-3816

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The abundance of oil palm decanter cake (OPDC) is a problem in oil palm mills. However, this lignocellulosic biomass can be utilized for cellulase and polyoses production. The effectiveness of chemical and physical pretreatment in reducing the lignin content was studied by saccharification using a Celluclast 1.5 L and scanning electron microscope. Physicochemical pretreatment of OPDC with 1% (w/v) NaOH and autoclaving at 121°C for 20 min increased potential polyoses produced to 52.5% and removed 28.7% of the lignin content. The optimized conditions for cellulase production by a locally isolated fungus were a time of 120 h, a substrate of untreated OPDC, a spore concentration of 1 × 107 spore/mL, a temperature of 30°C, and a pH between 7.0 and 7.5. Trichoderma asperellum UPM1 produced carboxymethylcellulase (CMCase), β-glucosidase and filter paper activity (FPase) in the following concentrations: 17.35, 0.53, and 0.28 U/mL, respectively. Aspergillus fumigatus UPM2 produced the CMCase, β-glucosidase and FPase in the following amounts: 10.93, 0.76, and 0.24 U/mL. The cellulases from T. asperellum UPM1 produced 2.33 g/L of polyoses and the cellulases from A. fumigatus UPM2 produced 4.37 g/L of polyoses.

Item Type:Article
Keyword:Aspergillus fumigatus UPM2; Cellulase; Oil palm decanter cake (OPDC); Polyoses; Pretreatment; Trichoderma asperellum UPM1
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:The Korean Society for Biotechnology and Bioengineering
DOI Number:10.1007/s12257-011-0590-9
ID Code:22428
Deposited By: Nabilah Mustapa
Deposited On:07 Jun 2016 16:36
Last Modified:07 Jun 2016 16:36

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