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Improvement of medium composition for heterotrophic cultivation of green microalgae, Tetraselmis suecica, using response surface methodology

Azma, Mojtaba and Mohamed, Mohd Shamzi and Mohamad, Rosfarizan and Abdul Rahim, Raha and Ariff, Arbakariya (2011) Improvement of medium composition for heterotrophic cultivation of green microalgae, Tetraselmis suecica, using response surface methodology. Biochemical Engineering Journal, 53 (2). pp. 187-195. ISSN 1369-703X; ESSN: 1873-295X

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Abstract

Optimization of medium composition for the improvement of heterotrophic cultivation of green microalgae, Tetraselmis suecica, was performed using response surface methodology (RSM). Heterotrophic cultivation of T. suecica was conducted in total darkness using Walne medium formulated with natural sea water. Initially, the effect of two types of carbon source (glucose and sodium acetate) and various types of nitrogen source (peptone, yeast extract, meat extract, malt extract, urea, sodium nitrate and ammonium nitrate) on growth of T. suecica was studied. The concentration of medium component that was found to significantly influence the heterotrophic growth of T. suecica (glucose, peptone, yeast extract and meat extract) was further optimized using RSM. The medium that consists of 5.78 g/L glucose, 9 g/L peptone, 4.48 g/L yeast extract and 3.01 g/L meat extract was found optimal for heterotrophic cultivation of T. suecica. The final cell concentration (28.88 g/L) obtained in heterotrophic cultivation using this optimized medium was about 3 and 2 times higher than obtained in photoautotrophic culture (8.40 g/L) and non-optimized medium for heterotrophic cultivation (13.81 g/L), respectively. In addition, the cell yield based on glucose consumed (9.31 g cell/g glucose) was increased by about 3 times as compared to non-optimized medium (3.61 g cell/g glucose).

Item Type:Article
Keyword:Green microalgae; Tetraselmis suecica; Heterotrophic algae culture; Medium optimization; Response surface methodology
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Elsevier
DOI Number:10.1016/j.bej.2010.10.010
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.bej.2010.10.010
ID Code:22318
Deposited By: Najwani Amir Sariffudin
Deposited On:11 Nov 2014 14:43
Last Modified:09 Oct 2015 08:48

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