Effect of oil palm empty fruit bunch (OPEFB) particle size on cellulase production by Botryosphaeria sp. in solid state fermentation.

Locally isolated Botryosphaeria sp. showed the ablity to produce cellulases (FPase, CMCase and β-glucosidase) from oil palm empty fruit bunch (OPEFB) as substrate. Different particle sizes (0.25-0.3 mm, 0.42-0.6 mm, 0.84-1.0 mm and 5.0-10 mm) of OPEFB were investigated under solid state fermentation on the cellulase production. The highest production of FPase and β-glucosidase were obtained from OPEFB particle size of 0.42 - 0.60 mm with 3.261 ± 0.011 U/g and 0.115 ± 0.008 U/g, respectively. It was found that among the four different OPEFB particle sizes studied, particle size of 0.84 - 1.0 mm gave the highest activity of CMCase (8.134 ± 0.071 U/g). Highest concentration of reducing sugars produced in this experiment was 4.303 ± 0.095 mg/ml.

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