Keyword Search:


Bookmark and Share

An improved method for the detection of white spot syndrome virus (WSSV) by single-tube nested deoxyribonucleic acid amplification and a detection kit utilizing the method.

Mohamed Shariff Mohamed Din. (2007) An improved method for the detection of white spot syndrome virus (WSSV) by single-tube nested deoxyribonucleic acid amplification and a detection kit utilizing the method.

Full text not available from this repository.

Abstract

The method of detection employs a nucleic acid amplification process using PCR by first amplifying an outer WSSV genomic DNA fragment that may be present in extracted DNA of clinical specimen using an "external" set of two oligonucleotide primers. Sensitivity and specificity are then increased through a second round of nucleic acid amplification of an internal WSSV DNA setgment located within the amplified outer WSSV genomic DNA fragment using an "internal" set of two oligonucleotide primers. The amplification process with these nested primers also reduces background and non-specific amplification. A detection kit the above incorporating the above is disclosed.

Item Type:Patent
Keyword:Aquaculture; Diagnostics; White spot syndrome virus; Nucleic acid.
Faculty or Institute:Faculty of Veterinary Medicine
ID Code:20881
Deposited By: Zubaidah Iberahim
Deposited On:14 Jun 2013 10:22
Last Modified:14 Jun 2013 10:22

Repository Staff Only: Edit item detail