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Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method

Abdul Rahman Omar. (2010) Detection and distinguishing infectious bursal disease virus (IBDV) strains by molecular biology method.

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Abstract

The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transriptase-polymerase chain reaction. Two different primer combinations (Primer IF & IVIR) and (Primer IF & RCLA) and real-time polymerase chain reaction conditions were designed and optimized for rapid differentiation of very virulent and vaccine strains of IBDV based on detection of signatory threshold cycle (Ct) and melting temperature (Tm) values.

Item Type:Patent
Keyword:Virology; Bursal disease virus; Birds; Vaccine
Faculty or Institute:Faculty of Veterinary Medicine
Application Number:12/551,969
Filing Date:2005-11-07
Granted Date:2010-12-28
ID Code:20452
Deposited By: Nurul Ainie Mokhtar
Deposited On:28 Jun 2013 08:26
Last Modified:28 Apr 2015 20:51

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