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Novel lipase gene from Bacillus sphaericus 205y

Raja Noor Zaliha Raja Abd. Rahman. (2009) Novel lipase gene from Bacillus sphaericus 205y.

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Abstract

A biologically pure culture of Bacillus sphaericus strain 205Y capable of producing an organic solvent - tolerant lipase was isolated from soil samples via direct plating method using 1 (V/V) of either benzene, toluene, or mixture of benzene and toluene as their sole carbon source, ethyl benzene and P-xylene. The lipase gene was isolated via genomic library and sequenced. The production of lipase gene from Bacillus sphaericus strain 205Y is a novel gene. The lipase gene was cloned from Bacillus spaericus strain 205Y and the recombinant lipase was efficiently excreted into the culture medium using expression vectors bearing the lipase gene.

Item Type:Patent
Keyword:Bacillus sphaericus; Lipase; Benzene; Toluene
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Application Number:PI20040958
Filing Date:2004-03-18
Granted Date:2009-10-30
ID Code:20420
Deposited By: Suzila Mohamad Kasim
Deposited On:27 Jun 2013 16:23
Last Modified:27 Sep 2016 09:44

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