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Enzyme assay for metal-reducing enzyme

Mohd. Yunus Abd. Shukor. (2009) Enzyme assay for metal-reducing enzyme.

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Abstract

An improved enzyme assay system for screening potential molybdenum-reducing microbes from the environment for the purpose of bioremediation is described. Said method includes contacting said crude enzyme preparation from the organisms with either nicotinamide adenine dinucleotide reduced from (NADH) or nicotinamide adenine dinucleotide phosphate in reduced from (NADPH), as electron donor substrates and laboratory-prepared phosphomolybdate as the electron acceptor substrate. The resultant blue intensity is mesured colorimetrically using a spectrophotometer. Said laboratory-prepared phosphomolybdate comprises of a mixture of molybdate and phosphate in the ratio of 10 moles molybdate to 1 to 5 moles phosphate.

Item Type:Patent
Keyword:Enzyme
Corporate Creators:Universiti Putra Malaysia
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
ID Code:20405
Deposited By: Suzila Mohamad Kasim
Deposited On:21 May 2013 10:54
Last Modified:21 May 2013 10:54

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