The differences of the oviductal ampulla secretory cells during follicular and luteal phases in the Ewes-from SEM and TEM point of view.

A.R., Intan Shameha and A.B. , Zuki Z. and Azmi, T.I. and H., Wahid and Nordin, M.M. (2007) The differences of the oviductal ampulla secretory cells during follicular and luteal phases in the Ewes-from SEM and TEM point of view. In: 6th ASEAN Microscope Conference "A Nanoscience Approach In Commercialization'., 10-12 Dec 2007, Pahang, Malaysia..

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Abstract

The aim of the present study was to differentiate the ultrastructure of secretory cells in the ampulla region of the sheep oviduct during follicular and luteal phases of estrous cycle. Fourteen ewes were slaughtered either at the peak od follicular or luteal phase for sample collection. Ampulla were taken and processed accordingly for scanning and transmission electron microscopy. Blood samples were taken every alternate day for hormonal profiles analysis using RIA. During follicular phase, the population of the secretaory cells was lower as compared to the luteal phase, while the number of ciliated cells was higher. The secretory cells were rounded, turgid with intact microvilli but during luteal phase the surfaces were broken and some secretions were oozing out.From TEM, at follicular phase, the secretory cells have blunt processes at the apex with intact microvilli, but during luteal phase, the cytoplasmic protrusion increased in volume. Numerous secretory granules were found in boh phases but they were different in sizes and electron density. In conclusion, the present observations revealed marked cyclic changes and difference of the secretory cells during these two phases of estrous cycle. During follicular phase, the secretory cells were at the preparatory stage while they were actively secreting during luteal phase.

Item Type:Conference or Workshop Item (Paper)
Subject:Sheep.
Subject:Ewes.
Subject:Female livestock.
Faculty or Institute:Faculty of Veterinary Medicine
ID Code:18998
Deposited By: Erni Suraya Abdul Aziz
Deposited On:16 Aug 2012 10:52
Last Modified:24 Dec 2013 01:33

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