Spectroscopic and microscopic evidence for adsorption of lipase from Candida rugosa onto calcined layered double hydroxides
Md. Yunus, Noor Mona and Abdul Rahman, Mohd Basyaruddin and Basri, Mahiran and Hussein, Mohd Zobir and Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha (2007) Spectroscopic and microscopic evidence for adsorption of lipase from Candida rugosa onto calcined layered double hydroxides. In: Enzyme Engineering XIX, 23-28 Sept. 2007, Columbia, Canada.
Lipase from Candida rugosa (CRL) was adsorbed onto three calcined layered double hydroxide (CLDH): Mg/A1-NO3- (CMAN) and Zn/A1-NO3- (CZAN). Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy (SEM-EDX) and protein staining light microscopy analyses were applied to provide direct spectroscopic and microscopic evidence for the adsorption of CRL onto CLDH. SEM-EDX spectra show CRL presence in CMAN-CRL, CNAN-CRL and CZAN-CRL prepared. Blue spots on the surface of CMAN-CRL,CNAN-CRL and CZAN-CRL stained with Coomasie Blue R confirm protein adsorption onto supports. The catalytic activity of all three CLDH-CRL was also evaluated in synthesis of methyl caprylate and results showed that CMAN-CRL and CNAN-CRL exhibited 70% conversion while CZAN-CRL exhibited 80% conversion compared to free lipase that exhibited only 6% conversion only.
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