Abdullah, Mohd Azmuddin and Ariff, Arbakariya and Mahmood, Maziah and Ali, Abdul Manaf and Lajis, Md Nordin (2000) Growth and anthraquinone production of Morinda elliptica cell suspension cultures in a stirred-tank bioreactor. Journal of Agricultural and Food Chemistry, 48 (9). pp. 4432-4438. ISSN 0021-8561
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Official URL: http://dx.doi.org/10.1021/jf991014v
The effects of medium strategy, number of impellers, aeration mode, and mode of operation on Morinda elliptica cell suspension cultures in a stirred-tank bioreactor are described. A lower number of impellers and continuous aeration contributed toward high cell growth rate, whereas a higher number of impellers reduced cell growth rate, although not anthraquinone yield. The semicontinuous mode could indirectly imitate the larger scale version of production medium strategy and improved anthraquinone production even with 0.012% (v/v) antifoam addition. Production medium promoted both growth (maximum dry cell weight of 24.6 g/L) and anthraquinone formation (maximum content of 19.5 mg/g of dry cell weight), without any necessity for antifoam addition. Cultures in production medium or with higher growth rate and anthraquinone production were less acidic than cultures in growth medium or with lower growth rate and anthraquinone production. Using the best operating variables, growth of M. elliptica cells (24.6 g/L) and anthraquinone yield (0.25 g/L) were 45% and 140%, respectively, lower than those using a shake flask culture after 12 days of cultivation.
|Keyword:||Anthraquinone; Bioreactor; Colorant; Medium strategy; Morinda elliptica; Plant cell culture|
|Subject:||Morinda - Biotechnology - Malaysia|
|Subject:||Plant tissue culture|
|Faculty or Institute:||Faculty of Biotechnology and Biomolecular Sciences|
|Deposited By:||Azwana Abdul Rahman|
|Deposited On:||05 Nov 2011 19:30|
|Last Modified:||05 Nov 2011 19:30|
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