Establishment of Physalis minima hairy roots culture for the production of physalins

Ganzau, Jualang Azlan and Mahmood, Maziah and Muse, Radzali and Ramli, Johari (2002) Establishment of Physalis minima hairy roots culture for the production of physalins. Plant Cell, Tissue and Organ Culture, 69 (3). pp. 271-278. ISSN 0167-6857

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Official URL: http://dx.doi.org/10.1023/A:1015662118877

Abstract

This report describes the technique used to induce the hairy roots in Physalis minima (Linn.). Different types of explants obtained from in vitro germinated seedlings were aseptically co-cultivated with A. rhizogenes strain LBA9402 in different media. Root growth and production of physalins were investigated in various basal media grown under dark and light conditions, and compared to that of normal root cultures. Transformed hairy root cultures grew rapidly and reach stationary phase after 15 days on a B5 medium. HPLC analysis of extracts of hairy root cultures showed that the maximum content of physalin B and F was 1.82 and 4.15 mg g-1 DW, respectively, when grown under dark conditions. Normal root cultures produced higher physalin B (1.60-1.62 mg g-1 DW) and F (3.30-3.75 mg g-1 DW) under the same culture conditions. Physalin F synthesis in light-grown root cultures was reduced significantly.

Item Type:Article
Keyword:Agrobacterium rhizogenes; Genetic transformation; Physalins production; Physalis minima; Transformed roots
Subject:Physalis - Genetics
Subject:Plant genetic transformation
Subject:Plant metabolites
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Springer
DOI Number:10.1023/A:1015662118877
ID Code:18376
Deposited By: Azwana Abdul Rahman
Deposited On:31 Oct 2011 09:44
Last Modified:31 Oct 2011 09:44

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