Ganzau, Jualang Azlan and Mahmood, Maziah and Muse, Radzali and Ramli, Johari (2002) Establishment of Physalis minima hairy roots culture for the production of physalins. Plant Cell, Tissue and Organ Culture, 69 (3). pp. 271-278. ISSN 0167-6857
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Official URL: http://dx.doi.org/10.1023/A:1015662118877
This report describes the technique used to induce the hairy roots in Physalis minima (Linn.). Different types of explants obtained from in vitro germinated seedlings were aseptically co-cultivated with A. rhizogenes strain LBA9402 in different media. Root growth and production of physalins were investigated in various basal media grown under dark and light conditions, and compared to that of normal root cultures. Transformed hairy root cultures grew rapidly and reach stationary phase after 15 days on a B5 medium. HPLC analysis of extracts of hairy root cultures showed that the maximum content of physalin B and F was 1.82 and 4.15 mg g-1 DW, respectively, when grown under dark conditions. Normal root cultures produced higher physalin B (1.60-1.62 mg g-1 DW) and F (3.30-3.75 mg g-1 DW) under the same culture conditions. Physalin F synthesis in light-grown root cultures was reduced significantly.
|Keyword:||Agrobacterium rhizogenes; Genetic transformation; Physalins production; Physalis minima; Transformed roots|
|Subject:||Physalis - Genetics|
|Subject:||Plant genetic transformation|
|Faculty or Institute:||Faculty of Biotechnology and Biomolecular Sciences|
|Deposited By:||Azwana Abdul Rahman|
|Deposited On:||31 Oct 2011 17:44|
|Last Modified:||31 Oct 2011 17:44|
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