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Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells

Tey, Beng Ti and Al-Rubeai, Mohamed (2005) Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells. Journal of Bioscience and Bioengineering, 100 (3). pp. 303-310. ISSN 1389-1723

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Official URL: http://dx.doi.org/10.1263/jbb.100.303

Abstract

Chemostat cultures of NS0 cell lines were carried out at dilution rates ranging from 0.8 d-1 to 0.2 d-1. Compared with the control, the viable cell density of the Bcl-2 cell line was approximately 10% higher at 0.8 d-1 and increased to 55% when the dilution rate was reduced to 0.2 d-1. As the dilution rate was reduced, the viability of the two cultures diverged reaching a difference of 43% at 0.2 d-1. The specific growth rate of the control cells was the same as the dilution rate down to a value of 0.6 d-1. By contrast, the specific growth rate of Bcl-2 cells was parallel to the dilution rate down to a value as low as 0.3 d-1. For both NS0 cell lines, the G1 cell population decreased, while the S and G2/M cell populations increased as the dilution rate was reduced. The antibody titer of the control cells increased from 7 to 21 μg·ml-1 as the dilution rate was reduced from 0.8 to 0.2 d-1. With an initial increase from 2 to 15 μg·ml-1 as the dilution rate was reduced from 0.8 to 0.4 d-1, the antibody titer of the Bcl-2 cells remained constant as the dilution rate was further reduced to 0.2 d-1. A good correlation between specific antibody production rate and the percentage of G2/M cells was observed. © 2005, The Society for Biotechnology.

Item Type:Article
Keyword:Apoptosis; bcl-2; Bioreactor; Chemostat culture; NS0 myeloma cells
Subject:Apoptosis
Subject:Cell culture
Subject:Animal cell biotechnology
Faculty or Institute:Faculty of Engineering
Publisher:The Society for Biotechnology
DOI Number:10.1263/jbb.100.303
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1263/jbb.100.303
ID Code:18322
Deposited By: Azwana Abdul Rahman
Deposited On:26 Oct 2011 05:30
Last Modified:26 Oct 2011 05:30

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