Genetic relatedness among isolates of Acanthamoeba based on RAPD analysis

Abdul Manaf, Siti Ruhaya and Mustafa, Shuhaimi and Mat Amin, Nakisah and Ali, Abdul Manaf (2006) Genetic relatedness among isolates of Acanthamoeba based on RAPD analysis. Journal of Applied Sciences, 6 (1). pp. 15-19. ISSN 1812-5654

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Acanthamoeba is a pathogen frequently infecting brain, eyes, skin and lung of human and animal. Seven Acanthamoeba local isolates from various sources, two Acanthamoeba type strains and one strain of Hartmannella vermiformis were characterized for their genetic variability using randomly amplified polymorphic DNA (RAPD) technique using four different 10-mer oligonucleotides primers. Electrophoresis of the amplification products generated DNA bands ranging from approximately 0.25 to 7.50 kbp in size. A genetic relatedness among the isolates was examined using Dice similarity coefficient as the genetic distance measured between the strains of Acanthamoeba and H. vermiformis. Three distinct clusters could be separated at genetic distance of approximately 0.330. © 2006 Asian Network for Scientific Information.

Item Type:Article
Keyword:Acanthamoeba; DNA polymorphism; Genetic distance; RAPD-PCR
Subject:Acanthamoeba - Malaysia
Subject:Genetic polymorphisms
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
DOI Number:10.3923/jas.2006.15.19
ID Code:18312
Deposited By: Azwana Abdul Rahman
Deposited On:25 Oct 2011 15:56
Last Modified:25 Oct 2011 15:56

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