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Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying

Abd Manap, Mohd Yazid and Shobirin, Anis and Mohamad, Rosfarizan and Mustafa, Shuhaimi and Wong, Stephenie and Kabeir, Barka Mohammed (2009) Viability of bifidobacterium pseudocatenulatum G4 after spray-drying and freeze-drying. Microbiology Insights, 2 (July). pp. 1-7. ISSN 1178-6361

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Viability of Bifidobacterium pseudocatenulatum G4 following spray-drying and freeze-drying in skim milk was evaluated. After spray-drying, the strain experienced over 99% loss in viability regardless of the air outlet temperature (75 and 85°C) and the heat-adaptation temperature (45 and 65 °C, 30 min). The use of heat-adaptation treatment to improve the thermotolerance of this strain was ineffective. On the other hand, the strain showed a superior survival at 71.65%–82.07% after freeze-drying. Viable populations of 9.319–9.487 log10 cfu/g were obtained when different combinations of skim milk and sugar were used as cryoprotectant. However, the addition of sugars did not result in increased survival during the freeze-drying process. Hence, 10% (w/v) skim milk alone is recommended as a suitable protectant and drying medium for this strain. The residual moisture content obtained was 4.41% ± 0.44%.

Item Type:Article
Keyword:Bifidobacteria; Freeze-drying; Cryoprotectant; Spray-drying; Survival; Viability
Subject:Skim milk
Subject:Dairy products
Faculty or Institute:Faculty of Food Science and Technology
Publisher:Libertas Academica Ltd.
DOI Number:10.4137/MBI.S2728
ID Code:17753
Deposited By: Khairil Ridzuan Khahirullah
Deposited On:05 Dec 2011 11:07
Last Modified:09 Nov 2012 09:34

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