Safety evaluation of Bifidobacterium pseudocatenulatum G4 as assessed in BALB/c mice

Kabeir, Barka M. and Abd.Manap, Mohd. Yazid and Wong, Stephenie Yoke Wei and Abdullah, Muhammad Nazrul Hakim and Othaman, Muhammad Anas and Mustafa, Shuhaimi (2008) Safety evaluation of Bifidobacterium pseudocatenulatum G4 as assessed in BALB/c mice. Letters in Applied Microbiology, 46 (1). pp. 32-37.

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Abstract

Aims:  To assess the safety of Bifidobacterium pseudocatenulatum G4 in BALB/c mice that involves examination of bacterial translocation, changes in the internal organs and histology of the intestinal lining. Methods and Results:  Forty male BALB/c mice were randomly assigned into five groups (n = 8). Three groups were orally fed with 50 μl of three different concentrations of B. pseudocatenulatum G4 (2 × 104, 1 × 108 and 1 × 1011 CFU day−1) for 4 weeks. One group was orally administered with 50 μl of 1 × 108 CFU B. longum BB536 per day for 4 weeks and last group was used as a nonbifidobacterial treatment control, which received 50 μl of skim milk. The administered strains did not affect the general health of mice and incapable of carrying out translocation to blood or liver. There were no significant differences in the internal organ (liver, heart, kidney and spleen) indices, serum enzymes of liver (aspartate aminotransferase, alkaline phosphate, alanine aminotransferase) and kidney (urea and creatinine) and histology (villi height, crypts height, mucosa thickness and epithelial cell height) of caecum, ileum and colon. Conclusion:  Administration of high dose of up to 1 × 1011 CFU B. pseudocatenulatum G4 per day to mice did not show any health threatening symptoms. Significance and Impact of the Study: Bifidobacterium pseudocatenulatum G4 is none pathogenic to BALB/c mice and could be safe probiotic for human consumption.

Item Type:Article
Keyword:Bifidobacterium, Infectivity, Marker enzymes, Organs, Safety, Translocation
Subject:Bifidobacterium
Subject:Mice - Infection
Faculty or Institute:Faculty of Food Science and Technology
DOI Number:10.1111/j.1472-765X.2007.02255.x
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1111/j.1472-765X.2007.02255.x
ID Code:17739
Deposited By: Nida Hidayati Ghazali
Deposited On:24 Aug 2011 04:46
Last Modified:24 Aug 2011 04:46

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