Immunomodulatory effects of damnacanthal isolated from roots of Morinda elliptica.

Mohamed Alitheen, Noorjahan Banu and Manaf, A. Abdul and Yeap, S.K. and Shuhaimi, M. and Nordin, L. and Mashitoh, A.R. (2010) Immunomodulatory effects of damnacanthal isolated from roots of Morinda elliptica. Pharmaceutical Biology, 48 (4). pp. 446-452. ISSN 1388-0209

Full text not available from this repository.

Abstract

Morinda elliptica Ridley (Rubiaceae) has been used traditionally as a medicine to treat various diseases in Malaysia and Southeast Asia. In the present study we investigated the immunomodulatory effects of damnacanthal isolated from the roots of Morinda elliptica. The immunomodulatory effect of this compound was evaluated by using the lymphocyte proliferation assay with mouse thymocytes and human peripheral blood mononuclear cells (PBMC). In addition, the effect of the compound on PBMC cell cycle progression was studied by using flow cytometry. The production of human interleukin-2 and human inteleukin-12 cytokines was also assessed using the enzyme linked immunosorbent assay (ELISA) technique. The lymphocyte proliferation assay showed that damnacanthal was able to activate mouse thymocytes and PBMC at a low concentration (0.468 μg/mL). Moreover, the production of human interleukin-2 and human interleukin-12 cytokines in the culture supernatant from damnacanthal activated lymphocytes was markedly up-regulated at 24 h and sustained until 72 h with a slight decrease with time. A positive correlation was found between the level of these two cytokines and the MTT-based proliferation assay. Based on the above results, damnacanthal can act as an immunomodulatory agent which may be very useful for maintaining a healthy immune system.

Item Type:Article
Keyword:Morinda elliptica; Damnacanthal; Immunomodulation; Interleukin.
Subject:Medicinal plants.
Subject:Materia medica, Vegetable.
Faculty or Institute:Faculty of Food Science and Technology
Publisher:Informa Healthcare
DOI Number:10.3109/13880200903168031
ID Code:17710
Deposited By: Khairil Ridzuan Khahirullah
Deposited On:05 Dec 2011 02:25
Last Modified:22 May 2013 07:10

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 05 Dec 2011 02:25.

View statistics for "Immunomodulatory effects of damnacanthal isolated from roots of Morinda elliptica."


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.