Siew, Ling Hii and Tau, Chuan Ling and Mohamad, Rosfarizan and Ariff, Arbakariya (2008) Enhancement of extracellular pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium based on sago starch. The Open Biotechnology Journal, 3 . pp. 1-8. ISSN 1874-0707
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Enhancement of pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium formulation was investigated in batch fermentation using 500-mL shake flask. The fermentations were carried out, firstly, to search for a suitable cultivation medium for enzyme production and followed by the evaluations on the influence of carbon and nitrogen sources and also initial culture pHs on the secretion of pullulanase by this bacterium. The modified mineral Czapek medium was found suitable to produce substantially high activity of pullulanase (320 times higher) as compared to ‘Ohba-Ueda’ medium. This bacterium was found superior in pullulanase production using sago starch and peptone as carbon and nitrogen sources, respectively. Using the optimized medium, the bacterium produced 0.95 U/mL of pullulanase at initial culture pH of 7 and incubation temperature of 30oC.
|Keyword:||Pullulanase; Raoultella planticola DSMZ 4617; Submerged fermentation; Sago starch.|
|Faculty or Institute:||Faculty of Biotechnology and Biomolecular Sciences|
|Publisher:||Bentham Science Publishers|
|Deposited By:||Nida Hidayati Ghazali|
|Deposited On:||30 Nov 2011 17:19|
|Last Modified:||01 Dec 2011 13:21|
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