Specific Interactions between Nipah Virus Nucleocapsid (N) Protein and Phospho-(P) Protein Using the Yeast Two-Hybrid System
Mohd Mohidin, Taznim Begam (2006) Specific Interactions between Nipah Virus Nucleocapsid (N) Protein and Phospho-(P) Protein Using the Yeast Two-Hybrid System. Masters thesis, Universiti Putra Malaysia.
Nipah virus (NiV) which is a member of a new genus, Henipavirus, in the family Paramyxoviridae, encodes an unusually large phospho- (P) protein compared to other known paramyxoviruses. In this study, the region(s) involved in the interaction between this exceptionally large P protein with its nucleocapsid (N) protein was investigated in vivo using the yeast two-hybrid system. Deletion analysis was used to map the domain(s) of both the N and P proteins involved in N-P and P-N interactions. Mapping of the domains of N protein involved in its interaction with the P protein revealed that the Cterminal 30 amino acids (423-452 residues) are crucial for N-P interaction. However, mapping of the domains of P protein involved in the P-N association demonstrated that both the C-terminal 63 amino acids (470-532 residues) and the immediate N-terminal 62 amino acids (1-62 residues) simultaneously play a major role. Comparison of these findings with other studies indicates that paramyxoviruses are different in terms of interaction domains(s) between these two essential viral proteins involved in genome replication.
Repository Staff Only: Edit item detail