Mohd Mohidin, Taznim Begam (2006) Specific Interactions between Nipah Virus Nucleocapsid (N) Protein and Phospho-(P) Protein Using the Yeast Two-Hybrid System. Masters thesis, Universiti Putra Malaysia.
Nipah virus (NiV) which is a member of a new genus, Henipavirus, in the family Paramyxoviridae, encodes an unusually large phospho- (P) protein compared to other known paramyxoviruses. In this study, the region(s) involved in the interaction between this exceptionally large P protein with its nucleocapsid (N) protein was investigated in vivo using the yeast two-hybrid system. Deletion analysis was used to map the domain(s) of both the N and P proteins involved in N-P and P-N interactions. Mapping of the domains of N protein involved in its interaction with the P protein revealed that the Cterminal 30 amino acids (423-452 residues) are crucial for N-P interaction. However, mapping of the domains of P protein involved in the P-N association demonstrated that both the C-terminal 63 amino acids (470-532 residues) and the immediate N-terminal 62 amino acids (1-62 residues) simultaneously play a major role. Comparison of these findings with other studies indicates that paramyxoviruses are different in terms of interaction domains(s) between these two essential viral proteins involved in genome replication.
|Item Type:||Thesis (Masters)|
|Chairman Supervisor:||Professor Datin Khatijah Binti Mohd Yusoff, PhD|
|Call Number:||FBSB 2006 11|
|Faculty or Institute:||Faculty of Biotechnology and Biomolecular Sciences|
|Deposited By:||INVALID USER|
|Deposited On:||23 May 2008 19:53|
|Last Modified:||27 May 2013 06:46|
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