Validated High Performance Liquid Chromatographic (HPLC) method for analysis of Zerumbone in plasma

M. Eid, Eltayeb Elamin and Abdul, Ahmad Bustamam and Al-Zubairi, Adel Sharaf and Sukari, Mohamed Aspollah and Abdullah, Rasedee (2010) Validated High Performance Liquid Chromatographic (HPLC) method for analysis of Zerumbone in plasma. African Journal of Biotechnology, 9 (8). pp. 1260-1265. ISSN 1684–5315

Full text not available from this repository.

Official URL:


Zerumbone (ZER) is a sesquiterpene derived from Zingiber zerumbet smith, family Zingiberaceae. It has been shown to possess anti-cancer and apoptosis-inducing properties against various human tumour cells as well as in vivo against a number of induced malignancies in mice. In this study a simple,specific and accurate high performance liquid chromatographic method for determination of ZER in micro-volumes human plasma (| 1.5 ml) was developed and validated. ZER and its analogue -Humulene as internal standard were easy recovered by simple one step plasma protein precipitation using acetonitrile and separated in isocratic mobile phase, on reverse phase-C18 column. The effluent was monitored by Photodiode Array (PDA) detector and at a flow rate of 1.0 ml/min. The linearity of proposed method was 2 – 15 μg/ml. The intra-day and inter-day coefficient of variation and percent error values of the method were less than 15% and mean recovery was more than 90% for both ZER and -Humulene. This method was found to be precise, specific, accurate and robust for detection and analysis of ZER in human plasma.

Item Type:Article
Keyword:Zerumbone; Humulene; HPLC; Human plasma.
Subject:High performance liquid chromatography
Subject:Chromatographic analysis
Faculty or Institute:Faculty of Science
Publisher:Academic Journals
ID Code:17571
Deposited By: Najwani Amir Sariffudin
Deposited On:15 Dec 2011 04:33
Last Modified:25 Aug 2014 11:33

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 15 Dec 2011 04:33.

View statistics for "Validated High Performance Liquid Chromatographic (HPLC) method for analysis of Zerumbone in plasma"

Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.