Validated High Performance Liquid Chromatographic (HPLC) method for analysis of Zerumbone in plasma

M. Eid, Eltayeb Elamin and Abdul, Ahmad Bustamam and Al-Zubairi, Adel Sharaf and Sukari, Mohamed Aspollah and Abdullah, Rasedee (2010) Validated High Performance Liquid Chromatographic (HPLC) method for analysis of Zerumbone in plasma. African Journal of Biotechnology, 9 (8). pp. 1260-1265. ISSN 1684–5315

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Official URL: http://www.academicjournals.org/AJB/PDF/pdf2010/22Feb/Eid%20et%20al.pdf

Abstract

Zerumbone (ZER) is a sesquiterpene derived from Zingiber zerumbet smith, family Zingiberaceae. It has been shown to possess anti-cancer and apoptosis-inducing properties against various human tumour cells as well as in vivo against a number of induced malignancies in mice. In this study a simple,specific and accurate high performance liquid chromatographic method for determination of ZER in micro-volumes human plasma (| 1.5 ml) was developed and validated. ZER and its analogue -Humulene as internal standard were easy recovered by simple one step plasma protein precipitation using acetonitrile and separated in isocratic mobile phase, on reverse phase-C18 column. The effluent was monitored by Photodiode Array (PDA) detector and at a flow rate of 1.0 ml/min. The linearity of proposed method was 2 – 15 μg/ml. The intra-day and inter-day coefficient of variation and percent error values of the method were less than 15% and mean recovery was more than 90% for both ZER and -Humulene. This method was found to be precise, specific, accurate and robust for detection and analysis of ZER in human plasma.

Item Type:Article
Keyword:Zerumbone; Humulene; HPLC; Human plasma.
Subject:High performance liquid chromatography
Subject:Chromatographic analysis
Faculty or Institute:Faculty of Science
Publisher:Academic Journals
ID Code:17571
Deposited By: Najwani Amir Sariffudin
Deposited On:15 Dec 2011 04:33
Last Modified:25 Aug 2014 11:33

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