Synbiotics growth optimization of Bifidobacterium pseudocatenulatum G4 with prebiotics using a statistical methodology

Mustafa, Shuhaimi and B.M., Kabeir and Abd. Manap, Mohd. Yazid and Abdullah, Muhammad Nazrul Hakim (2009) Synbiotics growth optimization of Bifidobacterium pseudocatenulatum G4 with prebiotics using a statistical methodology. Journal of Applied Microbiology, 106 (1). pp. 191-198. ISSN 1364-5072

Full text not available from this repository.

Abstract

Aims:  This study demonstrated the optimum growth of Bifidobacterium pseudocatenulatum G4 with prebiotics via statistical model.Methods and Results:  Commercial prebiotics [inulin and fructooligosaccharide (FOS)], together with sorbitol, arabinan and inoculum rate, were tested by fractional factorial design to determine their impact on growth of Bif. pseudocatenulatum G4 in skim milk. At 48 h incubation, bacterial growth was mainly influenced by FOS and inoculum rate. Growth reduction was observed in all samples incubated for 72 h. Central composite design (CCD) was adopted using FOS and inoculum rate at 48 h incubation to develop the statistical model for optimization. The model predicted that 2·461 log CFU ml−1 produced the optimum growth increase of Bif. pseudocatenulatum G4. The combination that produced the optimum point was 2·86% FOS (g/v) and 0·67% inoculum rate (v/v).Conclusion:  At optimum combination of inoculum rate and FOS, validation experiments recorded 2·40 ± 10·02 log CFU ml−1. The application in 1-l bioreactor for 24 h showed higher growth increase of 2·95 log CFU ml−1.Significant and Impact of the Study:  Response surface methodology approach is useful to develop optimum synbiotics combination for strain G4 with FOS.

Item Type:Article
Keyword:Bifidobacterium; Prebiotics; Response surface methodology; Synbiotic
Subject:Bifidobacterium
Faculty or Institute:Faculty of Food Science and Technology
Publisher:Wiley Blackwell
DOI Number:10.1111/j.1365-2672.2008.03991.x
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1111/j.1365-2672.2008.03991.x
ID Code:16917
Deposited By: Khairil Ridzuan Khahirullah
Deposited On:23 May 2012 03:56
Last Modified:31 Oct 2012 00:59

Repository Staff Only: item control page


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.