Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR.

Chai, Fung Pui and Woan, Chwen Wong and Chai, Lay Ching and Nillian, Elexson and Mohamad Ghazali, Farinazleen and Cheah, Yoke Kqueen and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2011) Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR. Food Control, 22 (2). pp. 337-342. ISSN 0956-7135

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Abstract

This study aimed to determine the prevalence and quantity of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits from hawker stalls and hypermarkets in Malaysia. Analysis was carried out using the most probable number (MPN) – multiplex polymerase chain reaction (PCR) method. The prevalence of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in 210 samples of sliced fruits examined were 23.3%, 7.6% and 3.8%, respectively with estimated quantity varying from 0 to 19 MPN/g. This study urged the authority to look into the biosafety of sliced fruits in Malaysia.

Item Type:Article
Keyword:Salmonella; Sliced fruits; Multiplex PCR; Simultaneous detection.
Subject:Salmonella food poisoning
Subject:Foodborne diseases - Prevention
Subject:Food - Microbiology
Faculty or Institute:Faculty of Food Science and Technology
Publisher:Elsevier
DOI Number:10.1016/j.foodcont.2010.05.021
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.foodcont.2010.05.021
ID Code:16726
Deposited By: Khairil Ridzuan Khahirullah
Deposited On:24 May 2012 03:15
Last Modified:22 Sep 2014 13:30

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