Sequence analysis and characterization of vacuolar-type H+-ATPase proteolipid transcript from Acanthus ebracteatus Vahl.

Ho, Chai Ling and Nguyen, Phuoc Dang and Harikrishna, Jennifer Ann and Abdul Rahim , Raha (2008) Sequence analysis and characterization of vacuolar-type H+-ATPase proteolipid transcript from Acanthus ebracteatus Vahl. DNA Sequence the Journal of DNA Sequencing and Mapping, 19 (1). pp. 73-77. ISSN 1042-5179

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Abstract

The vacuolar-type H+ -ATPase (V-ATPase) is a multimeric enzyme with diverse functions in plants such as nutrient transport, flowering, stress tolerance, guard cell movement and development. A partial sequence of V-ATPase proteolipid was identified among the expressed sequence tags (ESTs) generated from Acanthus ebracteatus, and selected for full-length sequencing. The 876-nucleotide cDNA consists of an open reading frame of 165 amino acids. The deduced amino acid sequence displays high similarity (81%) with its homologs from Arabidopsis thaliana, Avecinnia marina and Gossypium hirsutum with the four transmembrane domains characteristics of the 16 kDa proteolipid subunit c of V-ATPase well conserved in this protein. Southern analysis revealed the existence of several members of proteolipid subunit c of V-ATPase in A. ebracteatus. The mRNA of this gene was detected in leaf, floral, stem and root tissues, however, the expression level was lower in stem and root tissues.

Item Type:Article
Keyword:Vacuolar-type H+-ATPase proteolipid; Mangrove; Acanthus ebracteatus Vahl.
Subject:Plant enzymes.
Subject:Enzymes - Analysis.
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Taylor & Francis Group
ID Code:16672
Deposited By: Nida Hidayati Ghazali
Deposited On:16 Jul 2012 03:09
Last Modified:16 Jul 2012 03:09

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