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Semi and continuous anaerobic treatment of palm oil mill effluent for the production of organic acids and polyhydroxyalkanoates.

Hong, S.K. and Yoshihito, Shirai and Abdul Rahman , Nor Aini and Hassan, Mohd Ali (2009) Semi and continuous anaerobic treatment of palm oil mill effluent for the production of organic acids and polyhydroxyalkanoates. Resarch Journal of Environmental Science, 3 (5). pp. 552-559. ISSN 1819-3412

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Abstract

The aim of this study was to improve organic acids production in semi and continuous anaerobic treatment of Palm Oil Mill Effluent (POME) using a locally fabricated 50 L Continuously Stirred Tank Reactor (CSTR). The organic acids obtained were then used for polyhydroxyalkanoate (PHA) production by Ralstonia eutropha ATCC 17699 in a 2 L bioreactor. The conditions used in the anaerobic treatment were controlled pH 6.5, sludge to POME ratio at 1:1 and Hydraulic Retention Time (HRT) of 4 days. The organic acids obtained were about 15 g L-1 at steady state for both treatments and the organic acids yield (based on BOD) was 58.3%. The selected organic acids obtained were acetic (51.5%), propionic (27%) and butyric (21.5%) acid. The recovery of the clarified and concentrated organic acids from the treated POME was made using a two-steps evaporation process. The clarified organic acids (distillate) obtained were comprising of 44.6, 20.1 and 22.5 g L-1 acetic, propionic and butyric acids, respectively with a recovery of 76%. The organic acids collected were then used for polyhydroxyalkanoates accumulation by Ralstonia eutropha ATCC 17699 using pH stat fed-batch fermentation under nitrogen limitation of C/N 40 in a 2 L fermenter. The highest PHA concentration of 11.4 g L-1 (>90% w/w) was achieved in this process.

Item Type:Article
Keyword:Palm oil mill effluent; Organic treatment; Organic acids; Retention time; Polyhydroxyalkanoates; Distillation.
Subject:Palm oil.
Subject:Sewage - Purification - Anaerobic treatment.
Subject:Organic acids.
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Academic Journals
DOI Number:DOI: 10.3923/rjes.2009.552.559
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/rjes.2009.552.559
ID Code:16662
Deposited By: Nida Hidayati Ghazali
Deposited On:13 Jul 2012 16:03
Last Modified:15 Sep 2015 15:28

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