Screening for the optimal induction parameters for periplasmic producing interferon-a2b in Escherichia coli.
Azaman, Siti Nor Ani and Ramakrishnan, Nagasundara Ramanan and Joo, Shun Tan and Abdul Rahim , Raha and Abdullah, Mohd. Puad and Ariff , Arbakariya (2010) Screening for the optimal induction parameters for periplasmic producing interferon-a2b in Escherichia coli. African Journal of Biotechnology, 9 (38 ). pp. 6345-6354. ISSN 1684-5315
Screening for optimum induction parameters to improve the production of periplasmic interferon-2b(PrIFN-2b) by recombinant Escherichia coli was conducted using shake flask culture. Recombinant E.coli Rosetta-gami 2(DE3) harboring the plasmid pET26b containing IFN-2b gene under the control of the T7lac promoter was used, where the induction was accomplished by isopropyl -D-1- thiogalactopyranoside (IPTG). The induction parameters (inducer concentration, point of induction,induction temperature and the length of induction) were analyzed to find the suitable range to be used for further optimization process. From the analysis, narrow range of induction temperature from 16 to 30°C and IPTG lower than 2 mM were found suitable for induction of PrIFN-2b. On the other hand, early log phase was the preferred time to initiate the induction and the length of induction was dependent on the combination of other induction parameters used.
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