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Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein.

Lee, Khai Wooi and Tan, Wen Siang (2008) Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein. Journal of Virological Methods, 151 (2). pp. 172-180. ISSN 0166-0934

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Abstract

The recombinant hepatitis B virus (HBV) core antigen (HBcAg) expressed in Escherichia coli self-assembles into icosahedral capsids of about 35 nm which can be exploited as gene or drug delivery vehicles. The association and dissociation properties of the C-terminally truncated HBcAg with urea and guanidine hydrochloride (GdnHCl) were studied. Transmission electron microscopy (TEM) revealed that the dissociated HBcAg was able to re-associate into particles when the applied denaturing agents were physically removed. In order to evaluate the potential of the particles in capturing molecules, purified green fluorescent protein (GFP) was applied to the dissociated HBcAg for encapsidation. The HBcAg particles harbouring the GFP molecules were purified using sucrose density gradient ultracentrifugation and analysed using native agarose gel electrophoresis and TEM. A method for the encapsidation of GFP in HBcAg particles which has the potential to capture drugs or nucleic acids was established.

Item Type:Article
Keyword:Hepatitis B virus capsid; Denaturing agent; Capsid assembly; Hepatitis B core antigen; Green fluorescent protein; Protein encapsidation.
Subject:Hepatitis B virus.
Subject:Hepatitis associated antigen.
Subject:Green fluorescent protein.
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Elsevier
DOI Number:10.1016/j.jviromet.2008.05.025
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jviromet.2008.05.025
ID Code:16448
Deposited By: Nida Hidayati Ghazali
Deposited On:19 Jun 2012 07:14
Last Modified:19 Jun 2012 07:14

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