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Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein.


Citation

Lee, Khai Wooi and Tan, Wen Siang (2008) Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein. Journal of Virological Methods, 151 (2). pp. 172-180. ISSN 0166-0934

Abstract

The recombinant hepatitis B virus (HBV) core antigen (HBcAg) expressed in Escherichia coli self-assembles into icosahedral capsids of about 35 nm which can be exploited as gene or drug delivery vehicles. The association and dissociation properties of the C-terminally truncated HBcAg with urea and guanidine hydrochloride (GdnHCl) were studied. Transmission electron microscopy (TEM) revealed that the dissociated HBcAg was able to re-associate into particles when the applied denaturing agents were physically removed. In order to evaluate the potential of the particles in capturing molecules, purified green fluorescent protein (GFP) was applied to the dissociated HBcAg for encapsidation. The HBcAg particles harbouring the GFP molecules were purified using sucrose density gradient ultracentrifugation and analysed using native agarose gel electrophoresis and TEM. A method for the encapsidation of GFP in HBcAg particles which has the potential to capture drugs or nucleic acids was established.


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Additional Metadata

Item Type: Article
Subject: Hepatitis B virus.
Subject: Hepatitis associated antigen.
Subject: Green fluorescent protein.
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1016/j.jviromet.2008.05.025
Publisher: Elsevier
Keywords: Hepatitis B virus capsid; Denaturing agent; Capsid assembly; Hepatitis B core antigen; Green fluorescent protein; Protein encapsidation.
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 19 Jun 2012 07:14
Last Modified: 05 Oct 2015 08:28
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.jviromet.2008.05.025
URI: http://psasir.upm.edu.my/id/eprint/16448
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