Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia

Jasbeer, Kaura and Radu, Son and Mohamad Ghazali, Farinazleen and Cheah, Yoke Kqueen (2010) Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia. Food Control, 21 (11). pp. 1536-1544. ISSN 0956-7135

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Abstract

The present study which dealt mainly with processed feeds and some maize samples sold commercially in Malaysia evaluated the implementation of a real-time PCR cycling system for singleplex screening of eight target sequences (lectin, hmg, adh1, p35S, NK603, GA21, MON810 and MON863) and quantification of four genetically modified (GM) maize events (NK603, GA21, MON810 and MON863). The effects of using proprietary glass magnetic particles to bind DNA to their surface were also investigated in terms of DNA quantity, purity, integrity, quality and its overall effect on DNA amplification. GM material was present in 26.2% feeds and 65% maize samples. All GM samples contained MON810 followed by NK603 (47.5%), GA21 (25%) and MON863 (2.5%). Single-event and multiple-events were identified in the GM samples with 50% of the GM samples containing multiple-events. The present study which represents a fast and reliable methodology would provide an overview of the presence and levels of GMOs in feeds and maize in Malaysia.

Item Type:Article
Keyword:DNA; GMO; GMO quantification;Feeds; Real-time PCR
Subject:Corn - Genetic engineering - Malaysia
Subject:Genetically modified foods - Malaysia
Subject:Agricultural biotechnology
Faculty or Institute:Faculty of Food Science and Technology
Publisher:Elsevier
DOI Number:http://dx.doi.org/10.1016/j.foodcont.2010.03.018
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.foodcont.2010.03.018
ID Code:16440
Deposited By: Khairil Ridzuan Khahirullah
Deposited On:02 Jul 2012 01:46
Last Modified:18 Oct 2012 07:38

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