Real-time PCR evaluation of seven DNA extraction methods for the purpose of GMO analysis.

K., Jasbeer and Radu, Son and Mohamad Ghazali, Farinazleen and Cheah, Yoke Kqueen (2009) Real-time PCR evaluation of seven DNA extraction methods for the purpose of GMO analysis. International Food Research Journal, 16 (-). pp. 329-341. ISSN 1985-4668

Full text not available from this repository.


Successful DNA amplification is vital for the detection of specific DNA targets in feeds, and this in return depends on the ability of DNA extraction methods to produce good quality DNA. In this study, seven methods were compared for DNA extraction from feeds using quantitative polymerase chain reaction (PCR) of single copy maize (Zea mays) endogenous hmg (high mobility group) gene. Relative levels of hmg were used to evaluate the DNA quality. Spectrophotometer determination of DNA was also carried out to assess DNA yield and DNA purity, while electrophoretic analysis of genomic DNA extracts was carried out to investigate DNA integrity. The findings illustrate that the DNA extraction methods have a significant effect on DNA quality. Statistically, the Epicentre method extracted the highest DNA yield while the Wizard method had the lowest DNA yield with high DNA purity and integrity. However, the Wizard method recovered the most amplifiable DNA per reaction, indicating that template quality and integrity had greater influence over hmg amplification than DNA yield.

Item Type:Article
Keyword:DNA extraction; GMO; Real-time PCR; DNA amplification; Feeds.
Subject:Genetically modified foods - Analysis.
Subject:Polymerase chain reaction.
Subject:Gene amplification.
Faculty or Institute:Faculty of Food Science and Technology
Publisher:Faculty of Food Science and Technology,UPM
ID Code:16439
Deposited By: Khairil Ridzuan Khahirullah
Deposited On:02 Jul 2012 01:53
Last Modified:02 Jul 2012 01:53

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 02 Jul 2012 01:53.

View statistics for "Real-time PCR evaluation of seven DNA extraction methods for the purpose of GMO analysis. "

Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.