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Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia

Yusop, Zulkifli and Mohamed Alitheen, Noorjahan Banu and Radu, Son and Abdul Rahim, Raha and Lihan, Samuel and Yeap, Swee Keong and Nishibuchi, Mitsuaki (2009) Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia. International Food Research Journal, 16 (2). pp. 141-150. ISSN 1985-4668; ESSN: 2231-7546

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Official URL: http://www.ifrj.upm.edu.my/16%20%282%29%202009/03-...

Abstract

In this study, RAPD-PCR and ERIC-PCR were used to study the epidemiology of V. parahaemolyticus isolated from cockles in Padang, Indonesia. The Gold Oligo OPAR3 primer produced bands ranged from 1-8 with sizes from 0.2 – 5.0 kb and the Gold Oligo OPAR8 primer produced 1-7 bands with sizes 0.7 – 1.5 kb. Both primers produced twenty five RAPD patterns with a few isolates failed to produce any products. Based on phylogenetic dendrogram, all the isolates can be divided into 6 major clusters with similarity between 0 to 52%. For the ERIC primer, it produced bands ranged from 3-15 with sizes from 0.1 – 5.0 kb and twenty seven different ERIC patterns. Construction of the phylogenetic dendogram showed the isolates can be divided into 4 major clusters with similarity between 56 to 86%. The high diversity of both processes may be due to the multiple contamination sources of V. parahaemolyticus.

Item Type:Article
Keyword:V. parahaemolyticus; Seafood; RAPD-PCR; ERIC-PCR; Genomic fingerprinting
Faculty or Institute:Faculty of Food Science and Technology
Faculty of Biotechnology and Biomolecular Sciences
Publisher:Faculty of Food Science and Technology, Universiti Putra Malaysia
ID Code:16422
Deposited By: Khairil Ridzuan Khahirullah
Deposited On:14 Nov 2012 17:02
Last Modified:25 Sep 2015 08:45

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