Putative, phenylacetaldehyde synthase transcript of vanda mimi palmer: sequence and expression analysis.

Mohd-Hairul, Ab. Rahim and Chan, Wai Sun and Parameswari, Namasivayam and Gwendoline, Ee Cheng Lian and Abdullah, Janna Ong (2010) Putative, phenylacetaldehyde synthase transcript of vanda mimi palmer: sequence and expression analysis. International Journal of Botany, 6 (4). pp. 424-432. ISSN 1811-9700

Full text not available from this repository.

Abstract

Vanda Mimi Palmer is a fragrant-orchid of which has won several international awards for its fragrance emission characteristic. A partial sequence encoding putative phenylacetaldehyde synthase (VMPPAAS) transcript which might be involved in the fragrance biosynthesis of this orchid was previously identified in the floral Expressed Sequence Tags (ESTs) of this orchid. The aims of this study were to isolate full-length cDNA of VMPPAAS and followed by molecular characterization. The full-length cDNA was isolated by SMART RACE PCR amplification while the expression analysis was carried out using real-time RT-PCR for different tissues, different flower developmental stages and different time points in a 24 h cycle. The VMPPAAS transcript encodes for a polypeptide of 508 amino acid residues consisting of 1524 bp Open Reading Frame (ORF) flanked by 92 bp of 5' Untranslated Region (UTR) and 93 bp of 3' UTR including a poly-A tail. It has a conserved motif for pyridoxal phosphate attachment site and a VHVDAAY motif, which are also shared by other plant decarboxylase proteins such as tyrosine decarboxylases, tryptophan decarboxylases and phenylacetaldehyde synthase. Expression analysis of VMPPAAS transcript by real-time RT-PCR showed an up-regulated expression in floral tissues especially in sepal and petal and down-regulated in vegetative tissues. The expression was shown to be developmentally regulated in the flower life-cycle as well as temporally regulated in a 24 h cycle.

Item Type:Article
Keyword:Fragrant-orchid; Phenylacetaldehyde synthase; VMPAAS; Molecular chareacterization; Real-time RTPCR; Fragrance genes ; Orchid hybrid.
Subject:Orchidaceae (Orchid).
Subject:Flowers - Odor.
Subject:Aromatic plants - Analysis.
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Asian Network for Scientific Information
DOI Number:DOI: 10.3923/ijb.2010.424.432
ID Code:16399
Deposited By: Nida Hidayati Ghazali
Deposited On:16 Jul 2012 02:50
Last Modified:16 Jul 2012 02:50

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 16 Jul 2012 02:50.

View statistics for "Putative, phenylacetaldehyde synthase transcript of vanda mimi palmer: sequence and expression analysis."


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.