Protein expression of Late Elongated Hypocotyl (LHY) homolog genes of teak in escherichia coli.

Basherudin, Norlia and Muhammad, Norwati and Adnan, Norwati and Haron, Mohd Rosli and Mohd. Saleh , Norihan (2008) Protein expression of Late Elongated Hypocotyl (LHY) homolog genes of teak in escherichia coli. Pertanika Journal of Tropical Agricultural Science, 31 (2). pp. 257-261. ISSN 1511-3701

Full text not available from this repository.

Abstract

Expression of an isolated gene in a system that directly translates it into a protein is an important step to study the protein encoded by the gene. The isolated gene can be expressed in vivo by a heterologous system. In this study, a bacteria system was used to translate the Tectona grandis Late Elongated Hypocotyl (Tg-LHY) gene, which was isolated from flowering tissues of teak (Tectona grandis). The gene was cloned into the pET 14b vector (Novagen) and transformed into BL 21(DE3)/pLysS and Rosetta 2 expression host cells (Novagen). Rosetta 2 host cell has been found to be a good candidate to express the Tg-LHY protein from plant origin, as it recognizes the codon that was found in plant but rarely used in bacteria. The expressed protein was about an expected size, which was 90 kD. Western blot analysis using antibody against His-tag, which was fused to the Tg-LHY protein, proved that the expressed protein was Tg-LHY protein.

Item Type:Article
Keyword:Heterologous protein expression; Tectona grandis; LHY homolog genes.
Subject:Protein Engineering.
Subject:Gene Expression.
Subject:Escherichia coli - Genetics.
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Universiti Putra Malaysia Press
ID Code:16380
Deposited By: Nida Hidayati Ghazali
Deposited On:16 Jul 2012 03:00
Last Modified:16 Jul 2012 03:00

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 16 Jul 2012 03:00.

View statistics for "Protein expression of Late Elongated Hypocotyl (LHY) homolog genes of teak in escherichia coli."


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.