Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies.

Md Salleh, Madihah and Liew, Shiau Tsuey and Ariff, Arbakariya (2008) Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies. Biotechnology and Bioprocess Engineering, 13 (1). pp. 33-39. ISSN 1976-3816

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Abstract

The profile of enzymes relevant to solvent production during direct fermentation of sago starch by clostidium saccharobutylicum P262 in a 2 L stirred tank fermenter was determined utilizing different pH control strategies. During fermentation without pH control (initial pH of 6), the specific activity of crotonase, thiolase, and β-hydroxybutyryl-CoA dehydrogenase increased proportionally with solvent production. The highest crotonase (3,450.7 kat) and phosphotransbutyrylase activity(1,475.6 kat) was observed in fermentation where pH was maintained at 5 during the acidogenic phase and corresponded to a fairly high acid accumulation but low solvent production. During fermentation with a controlled pH of 5.25 during the solventogenic phase, the highest thiolase specific activity (255.7 kat) was obtained and corresponded to the highest production of acetone. On the other hand, the highest specific activities of crotonase, β-hydroxybutyryl-CoA dehydrogenase, and phosphotransbutyrylase were observed at pH 5.5 and corresponded to the highest production of ethanol and butanol. Butyryl-CoA dehydrogenase had no significance role in solvent fermentation. These results suggested that pH control strategies were important for improvement of solvent production during direct fermentation of sago starch by C.saccharobutylicum. © KSBB

Item Type:Article
Keyword:Sago starch; Clostridium saccharobutylicum; Solventogenesis; Acetogenesis; Enzymes relevant to solvent fermentation.
Subject:Sago.
Subject:Enzymes - Biotechnology.
Subject:Sago starch.
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:The Korean Society for Biotechnology and Bioengineering.
DOI Number:DOI/10.1007/s12257-007-0153-2
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s12257-007-0153-2
ID Code:16365
Deposited By: Nida Hidayati Ghazali
Deposited On:16 Jul 2012 04:23
Last Modified:16 Jul 2012 04:23

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