Senthil, Kumar Subramanian and Tey, Beng Ti and Hamid, Muhajir and Tan, Wen Siang (2009) Production of the matrix protein of nipah virus in escherichia coli: virus-like particles and possible application in diagnosis. Journal of Virological Methods, 162 (1-2). pp. 179-183. ISSN 0166- 0934
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The broad species tropism of Nipah virus (NiV) coupled with its high pathogenicity demand a rapid search for a new biomarker candidate for diagnosis. The matrix (M) protein was expressed in Escherichia coli and purified using a Ni-NTA affinity column chromatography and sucrose density gradient centrifugation. The recombinant M protein with the molecular mass (Mr) of about 43 kDa was detected by anti-NiV serum and anti-myc antibody. About 50% of the M protein was found to be soluble and localized in cytoplasm when the cells were grown at 30 degrees C. Electron microscopic analysis showed that the purified M protein assembled into spherical particles of different sizes with diameters ranging from 20 to 50 nm. The purified M protein showed significant reactivity with the swine sera collected during the NiV outbreak, demonstrating its potential as a diagnostic reagent.
|Keyword:||Matrix protein; Virus-like particles; Escherichia coli; Nipah virus; Paramyxovirus.|
|Subject:||Escherichia coli - Analysis.|
|Faculty or Institute:||Faculty of Biotechnology and Biomolecular Sciences|
|Deposited By:||Nida Hidayati Ghazali|
|Deposited On:||16 Jul 2012 02:57|
|Last Modified:||16 Jul 2012 02:57|
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