Production of the matrix protein of nipah virus in escherichia coli: virus-like particles and possible application in diagnosis.
Senthil, Kumar Subramanian and Tey, Beng Ti and Hamid, Muhajir and Tan, Wen Siang (2009) Production of the matrix protein of nipah virus in escherichia coli: virus-like particles and possible application in diagnosis. Journal of Virological Methods, 162 (1-2). pp. 179-183. ISSN 0166- 0934
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The broad species tropism of Nipah virus (NiV) coupled with its high pathogenicity demand a rapid search for a new biomarker candidate for diagnosis. The matrix (M) protein was expressed in Escherichia coli and purified using a Ni-NTA affinity column chromatography and sucrose density gradient centrifugation. The recombinant M protein with the molecular mass (Mr) of about 43 kDa was detected by anti-NiV serum and anti-myc antibody. About 50% of the M protein was found to be soluble and localized in cytoplasm when the cells were grown at 30 degrees C. Electron microscopic analysis showed that the purified M protein assembled into spherical particles of different sizes with diameters ranging from 20 to 50 nm. The purified M protein showed significant reactivity with the swine sera collected during the NiV outbreak, demonstrating its potential as a diagnostic reagent.
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