Potential cyclodextrin glycosyltransferase producer from locally isolated bacteria

Ramli, Norhayati and Abd. Aziz, Suraini and Hassan, Mohd Ali and Mohamed Alitheen, Noorjahan Banu and Kamaruddin, Kamarulzaman (2010) Potential cyclodextrin glycosyltransferase producer from locally isolated bacteria. African Journal of Biotechnology, 9 (43). pp. 7317-7321. ISSN 1684–5315

Full text not available from this repository.

Abstract

Cyclodextrin glycosyltransferase (CGTase) is one of the most important groups of microbial amylolytic enzymes that have been used for degradation of starch to yield cyclodextrin (CD) via cyclization reaction. The increasing demand for CD in industrial application has led to an extensive study about CGTase which begin with screening, isolation and characterization of CGTase-producing bacteria. The identification of CGTase producer involves the use of solid media containing phenolphthalein and methyl orange as indicators that was detected by the colour changes. The formations of clearance zone around the bacterial colony in the starch-containing medium were observed and the diameters were measured to gauge the hydrolytic efficiency of the bacteria. Out of 65 soil bacterial samples screened, Bacillus sp. NR5 UPM was identified as the most prolific CGTase producer, which produced highest CGTase activity (11.709 U/ml) and highest β ββ β-CD concentration (2.504 mg/ml) with α αα α-CD:β ββ β-CD:γ γγ γ-CD ratio was 0.5:91.1:8.4 when using raw soluble starch as a substrate. It also showed as the best CGTase producer when using sago starch as a substrate (15.514 U/ml). This isolate was known as a raw starch- degrading enzyme producer since it has the capability to use raw starch as a substrate. Thus, in the future, this new isolate perhaps can share the biggest market in industrial application.

Item Type:Article
Keyword:Cyclodextrin; Cyclodextrin Glycosyltransferase; Starch
Subject:Cyclodextrins
Subject:Glycosyltransferases
Faculty or Institute:Faculty of Food Science and Technology
Publisher:Academic Journals
DOI Number:10.5897/AJB10.081
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5897/AJB10.081
ID Code:16262
Deposited By: Khairil Ridzuan Khahirullah
Deposited On:27 Aug 2012 02:32
Last Modified:22 May 2013 07:12

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 27 Aug 2012 02:32.

View statistics for "Potential cyclodextrin glycosyltransferase producer from locally isolated bacteria"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.