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Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak


Citation

Sabullah, Mohd Khalizan and Rahman, M. F. and Ahmad, Siti Aqlima and Sulaiman, Mohd Rosni and Shukor, Mohd Shukri and Shamaan, Nor Aripin and Shukor, Mohd Yunus (2016) Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak. Agrivita, 38 (1). pp. 1-13. ISSN 0126-0537; ESSN: 2477-8516

Abstract

Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molybdenum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34oC. Glucose was the best electron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spectrum resembling a reduced phospho-molybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Faculty of Agriculture, University of Brawijaya, Indonesia
Keywords: Bioreduction; Glyphosate; Klebsiella oxytoca; Molybdenum blue; Molybdenum-reducing bacterium
Depositing User: Nabilah Mustapa
Date Deposited: 08 Jun 2016 01:15
Last Modified: 08 Jun 2016 01:15
URI: http://psasir.upm.edu.my/id/eprint/16206
Statistic Details: View Download Statistic

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