Isolation, Production and Characterization of Thermostable Lipase from Bacillus Sp. Strain L2

Mohammed Mukred, Abdul Daim (2004) Isolation, Production and Characterization of Thermostable Lipase from Bacillus Sp. Strain L2. Masters thesis, Universiti Putra Malaysia.

[img] PDF
185Kb

Abstract

A thermostable lipolytic endospore forming Bacillus sp. strain L2 was isolated from a hot spring (91oC) in Slim River, Perak, Malaysia. Nutritional studies showed that casamino acid was the best nitrogen source while trehalose and Tween 60 were the best carbon source and substrate respectively, for lipase production. The enzyme production was promoted in the presence of metal ions such as Ca2+, Cu2+, Fe2+, Co2+, Zn2+, and Sr2+. The optimum lipase production was obtained in the presence of CaCl2. Physiological study indicate that, this strain showed optimal lipase production after 28 h of incubation at 70 oC with 1% starting inoculum. The enzyme was purified to homogeneity by ammonium sulphate precipitation and affinity column chromatography (Heparin), with 3 fold increase in specific activity and 48.9 % recovery. The purified enzyme produced a single band on SDS-PAGE. Its molecular mass was estimated to be 43 KDa. The optimum pH and temperature for L2 lipase were determined to be 7.0 and 80 oC, respectively. The III lipase was very stable for 2 h at 80 oC. The stability at higher temperature was shown to be calcium dependent. The lipase activity was enhanced by Fe2+and Ca2+ while Cu2+ inhibited it. The enzyme activity was strongly inhibited by PMSF and completely inhibited by pCMB. Substrates such as olive oil and sesame oil enhanced the lipase activity.

Item Type:Thesis (Masters)
Subject:Lipase - isolation & purification
Subject:Biotechnology
Subject:Proteolytic enzymes
Chairman Supervisor:Associate Professor Md. Nordin Bin Lajis, PhD
Call Number:FBSB 2004 7
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
ID Code:16
Deposited By: Siti Khairiah Yusof
Deposited On:28 Apr 2008 21:15
Last Modified:27 May 2013 06:45

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 28 Apr 2008 21:15.

View statistics for "Isolation, Production and Characterization of Thermostable Lipase from Bacillus Sp. Strain L2"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.