Optimization of lipase-catalyzed synthesis of palm amino acid surfactant using response surface methodology (RSM).

Bidina, Hasmah and Basri, Mahiran and Mat Radzi, Salina and Ariff, Arbakariya and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar (2009) Optimization of lipase-catalyzed synthesis of palm amino acid surfactant using response surface methodology (RSM). Industrial Crops and Products, 30 (2). pp. 206-211. ISSN 0926-6690

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Abstract

Aminoacid surfactants are high-value surfactants which have excellent emulsifying characteristics and minimal toxicity to the living body. Enzymatic synthesis of palm kernel aminoacid surfactant was optimized by response surfacemethodology (RSM) using palm kernel olein (PKO) and l(+)-lysine catalyzed by Lipozyme RM IM. The reaction was performed in batch mode stirred tank reactor (STR) with one multi-bladed impeller. A central composite rotatable design (CCRD) was employed to evaluate the interactive effects of various parameters. The parameters were temperature (A): (40.00–70.00 °C), impeller speed (B): (100.00–400.00 rpm), substrates ratio (C): (1.00–4.00 mmol) and enzyme amount (D): (5.00–8.00 g). The optimum condition derived via RSM at fixed reaction time of 24 h was temperature; 47.50 °C, impeller speed; 323.96 rpm, substrates ratio; 3.25 mmol and enzyme amount; 7.25 g. The experimental yield was 89.03% under the optimum condition, which compared well with the maximum predicted value of 93.77%.

Item Type:Article
Keyword:Aminoacid surfactants; Palm kernel olein (PKO); Lipozyme; Stirred tank reactor (STR); Central composite rotatable design (CCRD).
Subject:Response surfaces (Statistics).
Subject:Palm oil - Analysis.
Subject:Lipase.
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Elsevier B.V.
DOI Number:http://dx.doi.org/10.1016/j.indcrop.2009.03.006
ID Code:15974
Deposited By: Nida Hidayati Ghazali
Deposited On:01 Aug 2012 00:44
Last Modified:01 Aug 2012 00:44

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