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Optimisation of lignin peroxidase production using locally isolated Pycnoporus sp. through factorial design


Citation

Zanirun, Zuraidah and Abd. Aziz, Suraini and Foo, Hooi Ling and Hassan, Mohd Ali (2009) Optimisation of lignin peroxidase production using locally isolated Pycnoporus sp. through factorial design. Biotechnology, 8 (3). pp. 296-305. ISSN 1682-296X; ESSN: 1682-2978

Abstract / Synopsis

Lignin peroxidase has been extensively studied and has been reported to produce by white rot fungus. The highest lignin peroxidase producer from local isolates, identified as Pycnoporus sp. was selected for the optimisation study. Factorial design approach was significant to determine the optimum conditions that significantly influenced the production of lignin peroxidase by Pycnoporus sp. Several factors were selected in a range indicated by -1 and +1 for lower and upper level, respectively. The results of ANOVA were analysed to check for the significant factors. Optimum condition for the highest lignin peroxidase activity of 51.1 U L-1 was obtained at 24 mM of nitrogen concentration, agitation speed at 110 rpm, pH 3.5, inoculum concentration of 6x106 spores mL-1 and with the addition of inducer (veratryl alcohol). Considering the results obtained, this statistical design was effective in improving the lignin peroxidase production from Pycnoporus sp.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: 10.3923/biotech.2009.296.305
Publisher: Asian Network for Scientific Information
Keywords: Ligninolytic; Lignin peroxidase; Optimise; Pycnoporus sp.
Depositing User: Nida Hidayati Ghazali
Date Deposited: 24 Jul 2012 16:17
Last Modified: 11 Aug 2017 17:49
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/biotech.2009.296.305
URI: http://psasir.upm.edu.my/id/eprint/15957
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